When a membrane is scanned perpendicularly to its surface, the fluorescence signal originating from the membrane itself must be separated from the signal of the surrounding medium for an FCS analysis. PyScanFCS interactively extracts the fluctuating fluorescence signal from such measurements and applies a multiple-tau algorithm. The obtained correlation curves can be evaluated using PyCorrFit.
Supported operating systems¶
- Windows 7 and higher
- Linux (Debian-based)
- Any other operating system with a Python 3.6 installation (via pip)
- Correlator.com Flex correlator (~.dat - photon stream data)
How to cite¶
Müller, P., Schwille, P., and Weidemann, T. Fluorescence Spectroscopy and Microscopy, chapter: Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane. Methods in Molecular Biology, Humana Press, Springer, New York. 2014, 635-651. doi:10.1007/978-1-62703-649-8_29 - download author’s manuscript.